Authors: Jiang C, Feng Y, Huang X, Xu Y, Zhang Y, Zhou N, Shen X, Chen K, Jiang H, Liu D Abeta(1-42) is the proteolytic cleavage product of cleavage of the amyloid precursor protein by beta- and gamma-secretases. The aggregation of Abeta(1-42) plays a causative role in the development of Alzheimer's disease. To lock Abeta(1-42) in a homogenous state, we embedded the Abeta(1-42) sequence in an unstructured region of Bcl-x(L). Both the N-terminus and the C-terminus of Abeta(1-42) were constrained in the disordered region, whereas the conjunction did not introduce any folding to Abeta(1-42) but maintained the sequence as a monomer in solution. With Bcl-x(L)-Abeta(42), we developed an enzyme-linked immunosorbent assay to compare the affinity of compounds for monomeric Abeta(1-42). Bcl-x(L)...